| 1. | None of the isolates from mainland china had multiple basic amino acid at ha cleavage site that confer high pathogenicity to some h5 and h7 atvs
流感病毒的血凝素ha蛋白是流感病毒的主要抗原之一，其抗体能中和流感病毒的感染，是主要的保护性抗体。 |
| 2. | The cleavage site of putative signal peptide was predicted to occur between 28 ( ala ) and 29 ( ala ) thus the putative mature form of the protein composed of 92 amino acids with a molecular weight of 9 . 2 kda
推测的最可能的信号肽切割位点位于第28 （ alal和29 （ alal个氨基酸之间。椎测的成熟肽共有92个氨基酸，分子量9 zkda 。 |
| 3. | Because of the cleavage site of enterokinase and cnbr was designed in the middle of thioredoxin and cmiv , the expressed peptides of the mutation of cmiv could be cuted down from the fusion protein by enterokinase or cnbr
由于硫氧还蛋白和抗菌肽之间设计了肠激酶( enterokinase )切割位点和cnbr切割位点，通过对该表达的融合蛋白的切割，可得到目标抗菌肽cmiv突变体多肽分子。 |
| 4. | Sequencing report of pcr product shows the ribozyme gene with two cleavage sites has already integrated into the genome of potato . and it also proved 35s promoter of prok2 was same as that of hajdukiewicz , p . and rna detection is going on
检测结果证明：所扩增的dna包含核酶基因反转录后的dna ，证明核酶基因已被转入马铃薯j - 1中；同时证明了转基因的prok2的35s启动子与hajdukiewicz ， p等所公布的启动子相同。 |
| 5. | Two amino acid substitutions near the vp2 - vp4 cleavage site were identified in the virulent strain jz2000 and might be involved in increased virulence of the virus . phylogenetic analyses indicated that these two strains were most closely related to some european strains but distinct from wibdv and variant strains
分子系统进化树分析表明， zj2000株和jd1株与cu - 1 、 p2 、 cef94 、 harbin等毒株的关系最近，而与欧洲、香港、日本的超强毒株和美国的变异株相对较远。 |
| 6. | 2 . the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein , encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids . 19 highly conserved , potential glycosylation sites and 17 cysteines residues were characterized with si protein , homology analysis showe that there were gene deletion -
S1基因：其全序列共1614bp (从起始密码子atg到s前体蛋白裂解位点) ，编码537个氨基酸，其氨基端有一编码18个氨基酸的信号肽序列，第12 13位氨基酸残基构成了信号肽的切割位点， 14 19位与111 124位氨基酸残基为s1蛋白的跨膜区域。 |
| 7. | The results indicate that the nucleotide sequences and deduced amino acid sequences of all the guangxi isolates in the signal peptides were highly homologous , but lowly homologous with other reference strains . the amino acid composes and arrangement of all guangxi isolates at the cleavage site has the typical pattern of ndv virulent strains , and is identical with the facts in the field cases . all the guangxi isolates are classified into genotype vii of apmv - 1 , the same genotype dominated in china and other areas in recent years
结果发现，广西分离株之间在信号肚的核旮酸和氨基酸同源性很高，而与其它参考株差异较大；广西分离株在裂解位点的氨基酸组成和排列均符合强毒株的特征，并与毒株在临床上的致病情况相符；根据apmvlf基因第47位第420位核苦酸序列所绘制的系谱树吵ylogenetictree ）来看，厂西鸡和鹅分离株都归属于基因型vll 。 |
| 8. | A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin . four oligonucleotide primers , based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1 , were designed to amplify specific dna fragment from different viruses
依据apmv - 1融合蛋白( f )基因裂解位点的核苷酸序列与其毒力的相关规律，分别设计合成了四条寡核苷酸引物，建立了一个可迅速检测不同禽源apmv - 1并可鉴定强、弱毒株的逆转录酶?聚合酶链式反应( rt - pcr )技术。 |
| 9. | The detection of ribozyme gene with two cleavage sites that cleaves plrv replicase gene were present in the second part of this paper . a conserved sequence of 35s promoter of plrv was found in gene pools . two primers were designed based on the conserved sequence and bamh i and ribozyme gene . the genomic dna of potato was amplified by the primers through polymerase chain reaction ( pcr )
从许多资料中报道的plrv的35s启动子的序列中找出一段保守序列，然后根据与核酶紧密相连的bamh和这段保守序列设计两段引物，用这两段引物通过pcr扩增转基因马铃薯的基因组dna ，并进行检测。 |
| 10. | The gene was 1668bp in length , encoding the f protein composed of 553 amino acids . sequence analysis and its secondary structure prediction showed that the f protein had three major hydrophobic regions consisting of about 25 amino acids , six potential glycosylation sites and thirteen cysteines . a sequence region of basic amino acids , rrqrrf , was found at the f , - f2 cleavage site , indicating that f4ge9 was a typical virulent strain
序列分析和二级结构预测表明， f _ ( 48 ) e _ ( 9 )株f蛋白含有3个分别由25个氨基酸组成的疏水区，存在6个潜在的糖基化位点， 13个半胱氨酸残基，裂解位点区域的氨基酸序列为rrqrrf ，说明f _ ( 48 ) e _ 9株是一株典型的强毒株。 |
